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After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)
Ageing, cellular senescence and chronic kidney disease: experimental evidence
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doi: 10.1016/j.abb.2017.09.009 119
FerrierLBrardFDebrauwerLChaboCLangellaPBunoLet alImpairment of the intestinal barrier by ethanol involves enteric microflora and mast cell activation in rodents
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