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The collected lysate can then be used immediately or at a later time following transportation and/or storage
a shearing homogenizer
After pooling by equal volume
This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt)
The unique spin-column design also provides zero buffer retention and a low elution volume
dsDNA Shearase Plus Size:50 U The collected lysate can thendsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5 phosphate and 3 hydroxyl termini. It has a particularly strong preference for dsDNA and generates random ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
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