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Environmental factors, stress, and aging also contribute to its decline

Temperature proves most critical - every 10C increase doubles degradation rate, meaning room temperature storage (25C) degrades peptides 4-8x faster than refrigeration (2-8C) and dramatically faster than freezing (-20C or below)

Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay

Ensure the patient understands the difficulty of the pathway and the need for support

Plant J 64:825838 Diaz-Vivancos P, Wolff T, Markovic J, Pallardo FV, Foyer CH (2010b) A nuclear glutathione cycle within the cell cycle