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This device released a metered dose of insulin into a chamber covering the participants nose, which was then inhaled by breathing regularly for 2 min until the prescribed dose was delivered

Methods Strains and medium The organisms used in this study are listed in Supplementary Table 3

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In UK clinical practice the standard injectable diluent is Water for Injections BP, and bacteriostatic water with benzyl alcohol is generally treated as a sterile supply rather than a medicine in its own right unless it is licensed for a specific indication