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10 minutes before the imaging, mice were anesthetized with isoflurane (Covetrus, 029405) and injected IP with D-luciferin potassium (MedChemExpress, HY-12591B) dissolved in PBS (150 mg/kg)

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Researchers should note that despite GHK-Cus favorable safety profile, the subcutaneous or intramuscular administration of peptides in general is associated with injection site pain, lightheadedness, nausea, and even flu-like symptoms in subjects

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