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Western blotting Total protein from the kidneys and HK-2 cells in each group collected using RIPA buffer (Thermo Fisher Scientific, Inc.), and the protein content was determined using the BCA method

Fact 03 GHK-Cu plasma levels fall from roughly 200 ng/mL at age 20 to under 80 ng/mL by age 60, a 60 percent decline that microinfusion can directly replenish at the dermal layer

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