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In comparison to the SLZ group, the Dula (50 g/kg) groups colonic sections had more positively stained crypts (Fig

After permeabilization with 0.5% Triton X-100, the samples were incubated with primary antibodies against FOXP3 (Invitrogen, lot 2,349,824), TNF- (Invitrogen, lot ZK4531382A), and IL-1 (Invitrogen, lot ZJ4505521)