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Figure 4 Alterations in the intracellular redox status are often associated with GSH depletion (Polonikov, 2020) and endogenous GSH deficiency, due either to decreased biosynthesis and/or increased consumption, is a marked contributor to the pathogenesis of numerous diseases via mechanisms including oxidative stress and inflammation

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After incubation for 1 h, the DCFH-DA probe (10 M) was added and incubated in the dark for 30 min to detect ROS