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To prepare the NbLumSyt1-HALO/JF549 complex, a 110-time molar excess of NbLumSyt1-HALO was mixed with JaneliaFluor 549 HaloTag ligand for super-resolution microscopy (Promega, GA1110) at room temperature (i.e., 22-25C) for 30 min, covered from light, in gentle rotation (20 rpm)
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Hydrolysis of Amides Using Aqueous Acid: Mechanism All this is to say that performing the hydrolysis of an amide is not nearly so easy as cleaving an acid halide
FOLIC ACID is involved in the formation of the amino acids glycine and methionine, as well as the vitamin choline