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The determination of early apoptosis was done at 24 h by aspirating the MM cells away from the BMSC and using Annexin V assay with flow cytometry and cytotoxicity at 96 h using DIMSCAN assay as previously described

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Refining colorectal cancer classification and clinical stratification through a single-cell atlas

Three-dimensional (3-D) model of vascular smooth muscle cells Rat vascular smooth muscle cells were maintained in high glucose DMEM supplemented with 10% fetal bovine serum