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Samples of small volume (150-200L) were thawed under strict cooling conditions (+0C to +2C) within 12-15 minutes in an ice-water bath preferentially without agitation

A pilot study showed that during a 6-hour IV NAD+ infusion, urine NAD+ levels jumped by 538%

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The average age of the unaffected children was 9.5 2.6 years, with samples taken from 17 Males and 14 Females