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Detection of protein carbonylation Levels of carbonylated proteins were determined with the OxyBlot protein oxidation detection kit following the manufacturers protocol (Millipore, Billerica, MA, USA)

This also applies to the mixing solution since using it cold can disrupt the process

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142 Activity-dependent mRNA translation and protein synthesis Several activity-regulated translational control pathways have been demonstrated to participate in pathologies of autism, such as the ERK/MAPK (mitogen-activated protein kinase) 143 and PI3K/mTOR (mammalian target of rapamycin) pathways