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Whole cell radioligand binding assay Cells were seeded at a density of 30,000 cells/well into 96-well culture plates and incubated overnight in DMEM containing 5% FBS at 37C in 5% CO 2

Growth Horm IGF Res 11:247249 Frane J, Bailly J, Chernausek SD, Kuntze J (2006) Growth responses to higher dose recombinant human IGF-I (rhIGF-I) in children with severe primary IGF-I deficiency (IGFD) due to growth hormone insensitivity (GHIS) (abstract P03-619)

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