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Desalted peptides were separated on an EASY-Spray PepMap C18 column (50 cm 50 m ID, 2 m particles, 100 pore size) using a linear gradient of 5% to 35% buffer B (0.5% formic acid in 80% acetonitrile)

After receiving the insulin, the subjects were asked to sniff to aid in the delivery of the drug into the nasal cavity

This allows you to monitor how your body reacts without flooding your system