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Immunofluorescence For whole-mount staining, the mouse esophagus was opened longitudinally, the muscle layer was removed and the epithelium was incubated for 3 h in 5 mM EDTAPBS buffer at 37 C

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Just as a building requires high-quality cement, steel, and other fundamental materials, the tissues need essential elements such as protein, vitamins, and minerals for self-repair after surgery

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