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Assessment of cell differentiation The differentiated C2C12 cells were stained with Crystal Violet Staining Solution (Beyotime, China), and images were taken using an inverted microscope (Nikon ECLIPSE Ti, Japan)

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They analyzed data from The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Gene Expression Omnibus (GEO) databases to explore DPP-IV expression, its impact on THCA prognosis, and its connection to immune response