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However, high level of ROS (Guan et al., 2015), caused by damage to poly unsaturated fatty acids of the cell membrane and break the disulfide bonds in proteins, that cause an accumulation of unfolded proteins in the ER

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Dot blot overlay To demonstrate the biological activity of the purified recombinant proteins, an overlay assay was performed wherein increasing concentrations of purified 6XHis-tagged Cr FAP174 protein was spotted directly onto a nitrocellulose membrane