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Myocardial ischemia-reperfusion injury, antioxidant enzyme systems, and selenium: A review

10.1371/journal.pmed.1003583

The assay method is based on monitoring the generation of GSH from glutathione disulfide (GSSG) by the action of glutathione reductase in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH)

R NQO1 Substrates NQO1 processes a wide range of substrates: Benzoquinones (from benzene metabolism, industrial exposure) Catechol estrogen quinones (from estrogen metabolism by CYP1A1/CYP1B1) CoQ10 (ubiquinone to ubiquinol reduction) Menadione (vitamin K3) Mitomycin C and other quinone-containing chemotherapy drugs Naphthoquinones Polycyclic aromatic hydrocarbon (PAH) quinones (from cigarette smoke, grilled food, air pollution) NQO1 and NRF2 NQO1 gene expression is controlled by the antioxidant response element (ARE) in its promoter region, which is activated by the transcription factor NRF2

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