BENTONVILLE, Ark
According to the FDAs brief , the concern was whether the stability of the peptide would hold during manufacturing and how much the quality of the compounded product could change as a result
But any temperature excursion above 8C for more than 2 hours causes irreversible protein denaturation
Solomkin JS, Mazuski JE, Baron EJ, Sawyer RG, Nathens AB, DiPiro JT, Buchman T, Dellinger EP, Jernigan J, Gorbach S, Chow AW, Bartlett J, Infectious Diseases Society of A (2003) Guidelines for the selection of anti-infective agents for complicated intra-abdominal infections
Next, proteins were treated with 3C-HRV protease (Genescript, Z03092) overnight at 4 C without shaking according to the manufacturers instructions