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It is not usually recommended for children or pregnant women without doctor advice

Staff writers Alison McCook and Harold Brubaker contributed to this article.

Chen, D.-B

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Cells were suspended in MEM, centrifuged for 10 min at 100 g then cultured in medium (MEM [Gibco], 10% FBS [Gibco], 4 mM L-glutamin [Sigma] + 2.5 g/ml amphotericin B [Gibco], 0.04 mg/ml Gentamicin [Gentamicin Sandoz 80 mg/ml]) for 14 days at 37 C, 5% CO 2