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The proteins were then transferred onto a PVDF membrane and corresponding SNAP/FLAG-hGLP-1R levels detected by Western blotting with an anti-SNAP antibody (P9310S, New England Biolabs)
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The large number of fragments produced during collision-induced dissociation (CID) further dilute the intensity in MRM mode [7,8]
There are also many multi-target dual agonists under development, such as GLP-1R/GCGR, GLP-1R/fibroblast growth factor 21 receptor (FGF21R), etc
In short, GLP-1 RA users had longer diabetes duration, higher prevalence of obesity, and higher prevalence of previous use of insulins and sulfonylureas than those in the DPP-4i group
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