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For each z-slice, a relative signal was obtained by calculating the ratio intensity (mean gray value) for Ubx signal (488 nm) relative to the DAPI signal in T2-T3 segments (which only expressed ectopic Ubx protein) using the same region of interest (ROI)

10.4 Ocular delivery barriers and potential side effects The bloodretinal barrier, composed of tight junctions in retinal vascular endothelial cells and the RPE, hinders the penetration of macromolecular drugs (such as iron chelators) into the posterior segment of the eye (181)