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Colon-colon anastomosis complicated by the cysteamine-induced colitis Under deep anesthesia the cysteamine (400 mg/kg) was applied through enema (1 ml/rat) at 8 cm proximal to the anus (as described before) (5-7, 9) and 10 minutes thereafter, the colon-colon anastomosis was created 5 cm proximal to anus

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Additional evaluations, including the JC-1 assay, DCFH-DA staining, and mtDNA analysis, were performed to assess its effects [158]