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Briefly, a small portion of red gastrocnemius muscle was separated and incubated in buffer X [7.23mM K 2 EGTA, 2.77 mM CaK 2 EGTA, 20 mM imidazole, 20 mM taurine, 5.7 mM ATP, 14.3 mM phosphocreatine, 6.56 mM MgCl 2 6H 2 O, and 50 mM 2-(N-Morpholino) ethane sulfonic acid potassium salt (K-MES) (pH7.4)]

Constipation is a possible side effect of many medications, including Topamax

Clinical trials would need an IND protocol

Supplementary Table 4 Adverse events by baseline eGFR