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Significance between the groups was analyzed by Two-way ANOVA followed by Bonferroni post test, using GraphPad Prism 6 software

Nishiyama, K

Due to intrinsic Cpf1 ribonuclease activity that facilitates crRNA processing and an array of donor DNA sequences for homology-directed repair of double-strand breaks generated by Cpf1, the multi-CRISPR/Cpf1 system is more efficient for multiplex deletion of up to four genes (Jimnez et al., 2019a)

-galactosidase activity was detected using X-Gal staining solution (1 mg ml 1 X-gal, Thermo Scientific, 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 )) diluted in 1 mM MgCl 2 /PBS (pH 5.56) for 818 h at 37 C