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The wells were blocked with DPBS (pH 7.4)/4% (w/v) skimmed milk powder (Sigma-Aldrich) (PBS/M) (250 L/well) for 1 h at room temperature and washed three times with PBS/0.05% (v/v) Tween 20 (PBS/T) at either pH 5.5 or pH 7.4

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This binding also relies upon transmembrane serine protease 2 (TMPRSS2) and furin peptidase, which mediate the cleavage of the spike protein at the S2 site and S1/2 sites, respectively (14, 15)

Second, evaluate other factors that influence hunger, such as stress, sleep quality, hydration, and meal composition