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Briefly, mostly ring-stage parasites were incubated at 0.2% initial parasitemia and 1% hematocrit with a range of CB-27 concentrations at 37C for 72 h in 96-well plates as previously described ( 50 was calculated using GraphPad Prism 6 software

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Science check: While human studies exist, they are limited, but the biochemical rationale is strong, and NAC is well-studied for liver protection in other contexts (like acetaminophen overdose)