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Additionally, in vitro ubiquitination assays using purified dimeric DDD-Ube2e2-COP1-c-Jun complexes showed c-Jun polyubiquitination in the presence of ATP, E1, Ube2d3 and neddylated CULLIN4-RBX1 (Supplementary Fig

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5 (download XLSX Numerical data for absorption and fluorescence spectra, quantification of GSH or roGFP ratio in cells, and near-infrared ratiometric imaging