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The researchers utilized the Achilles functional index (AFI), myeloperoxidase activity, histological inflammatory cell influx, and vascular index as potential markers

To test the requirement for copper in the media, cells were grown in the absence of copper for 48 h (the earliest observed time of maximum GspD depletion (Fig

For gene expression analysis, 1 g of RNA was used for cDNA synthesis using qScript cDNA Synthesis Kit (Quanta Bio, #66196756)

Interestingly, none of the drug incubations tested led to oxidation of the redox protein thioredoxin 1

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