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They took gout patients and maintained their iron stores at the lowest level possible without causing anemia andgouty attacks markedly diminished in every patient, with effects ranging from a complete remission to a marked reduction of incidence and severity of gouty attacks

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Bacterial pellets were obtained by centrifugation for 15 min at 3,220 g and pellets were resuspended in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)

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