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Following the final centrifugation at 160 g for 3 minutes, the cell pellet was resuspended, counted, and replated at a concentration of 1 x 10 5 cells/cm 2 in two of the four tested conditions: (1) N2B27 medium (see above) containing 10 g/ml BDNF (Qkine) (NB) and (2) co-cultures of hPSC-derived neurons with primary mouse astrocytes in N2B27 medium (NBA)

doi: 10.1038/s41598-022-20613-w 67 SharifTMartellEDaiCGhassemi-RadMSLeeKSinghSKet al

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However, its important to note that individual responses can vary, and these medications should be used as part of a comprehensive treatment plan that includes lifestyle changes