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Precipitated peptide was recovered by filtration and washed three times with ice-cold ether

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This buffer ratio varies with the pH, but owing to its relatively low concentration, it contributes little to the buffering capacity of the extracellular fluid

Peptide Sciences+1 NNMT is a cytosolic enzyme that catalyzes methyl transfer from S-adenosyl methionine (SAM) to nicotinamide and related substrates