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Thin sections were cut using an ultramicrotome (Leica Microsystems, Germany), and the sections were subjected to double staining with uranyl acetate and lead citrate and then observed using a transmission electron microscope (Hitachi HT7800, Japan)

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Serum hepatitis B core-related antigen (HBcrAg) correlates with covalently closed circular DNA transcriptional activity in chronic hepatitis B patients

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