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The dose of colistin was chosen based on a preliminary experiment to determine the dose sufficient to lead to histological damage in all treated mice

Next, the supernatant was obtained by carefully removing the lipid layer and was centrifuged again at 15,000 g for 20 min at 4 C to ensure all lipid phase removal

Before use, the solution in the ampoule should be carefully examined for any signs of discoloration or cloudiness, and questionable solutions should be discarded

It is possible that the amount of muscle damage and inflammation was so great during this study that administration of antioxidants had no possible benefit or as noted could possibly have had enhanced the response[27]

It's also important to consult a healthcare professional before making significant dietary changes