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One- or two layer density gradient centrifugation alone may not be sufficiently discriminating to provide a pure preparation of Kupffer cells, but this technique can provide an important initial enrichment for these cells prior to the use of centrifugal elutriation, adherence of the Kupffer cells to a plastic surface

P values were derived from two-sided unpaired t-test ( b ), two-sided Pearsons chi-square tests ( f ), One-way ANOVA ( c,d,g,h,j,i )

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A reasonable target for most users falls between 1200-1800 calories daily for women and 1500-2200 calories daily for men, adjusted based on activity level and rate of weight loss

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