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Cells were maintained in DMEM (HCT116, SW480, U2OS), MEM- with 1 ng ml 1 fibroblast growth factor 2 (Peprotech, hMSC) or DMEM with 1% non-essential amino acid (Gibco) and 50 g ml 1 uridine (Sigma-Aldrich) supplementation (143B), with 10% dialysed foetal bovine serum (dFBS), 50 U ml 1 penicillin and streptomycin (ThermoFisher, 15070063) and 1 mM sodium pyruvate (ThermoFisher, 11360070)

Mitochondrial dysfunction: at the nexus between alcohol-associated immunometabolic dysregulation and tissue injury

Drinking straight alcohol or alcohol mixed with carbonated beverages speeds up absorption

Emergency scenarios Power outage Powder peptides : Can survive days to weeks at room temp, will be fine

The process of mixing bac water with peptides requires precision, and having the right tools for the job is essential