(A) Schematic showing BirA specifically expressed in the germline (in green) biotinylating LAG-1 containing the BioTag
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The solvent was evaporated in vacuo to give a crude mixture that was further purified by HPLC (water and acetonitrile, both supplemented with 0.1% trifluoroacetic acid) on a Cosmosil C18 AR-II column (5.0 m, 10 250 mm