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To minimize oxidation, prepare working solutions immediately before use and avoid prolonged exposure to air

[DOI] [PMC free article] [PubMed] [Google Scholar] 41.Seif Eldin WR, Saad EA, Monier A, Elshazli RM

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This is because, upon heating, the compact native form of BSA becomes more flexible and reactive, exposing the Tyr and Trp residues from the hydrophobic core of BSA molecule to a more polar solvent environment 64,65