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The first portion was directly homogenized in ice-cold 10 mM phosphate buffer (pH 7.4) to prepare a 10% (w/v) homogenate, which was centrifuged at 5000 rpm for 10 min at 4 C

[DOI] [PMC free article] [PubMed] [Google Scholar] 289.Klein E.A., Thompson I.M., Tangen C.M., Crowley J.J., Lucia M.S., Goodman P.J., Minasian L.M., Ford L.G., Parnes H.L., Gaziano J.M., et al

Cagrilintide is a 39amino-acid peptide with structural modifications (including an N-terminal fatty diacid chain) designed to improve stability and reduce fibril formation compared with native amylin

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Some quiet hunger, while others block fat or change hormone signals