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Maintenance treatments may be recommended in some cases to sustain the results for longer periods

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Peridural membrane and circumneural sheath

Occupational social grade was categorised using National Readership Survey classifications [32] as ABC1 (includes managerial, professional, and upper supervisory occupations) and C2DE (includes manual routine, semi-routine, lower supervisory, state pension, and long-term unemployed)

cAMP accumulation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were diluted to the density of 6 10 5 cells/ml and INS-1(832/13) cells to 2 10 6 cells/ml in stimulation buffer [phenol-free DMEM containing 5 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) and 0.1% (w/v) BSA], and cAMP detection was carried out as described previously 48