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Cells were then washed three times for 10 min each in CACO buffer

In the case of the Russian research, they started with extracts from the thymus (left) and pineal glands (right)

Hence, in a fully-recovered receptor, the four LBDs should rearrange into two dimers as observed previously in GluK2 31 and GluA2 28,29 closed-state structures to enable coupling of agonist binding with channel opening 36

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