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Bacteriostatic water goes in slowly down the side of the vial rather than blasted straight onto the powder, and the vial is swirled, not shaken, until the solution turns clear

For all practical lab calculations, you can assume the final volume of the solution is equal to the volume of the bacteriostatic water you added

However, in addition to the advanced instrumentation and analytical techniques, the quality and behavior of the biological samples remain crucial for successful structure determination using both methods, with flexibility and conformational dynamics being the usual reasons for decreasing resolution

Wipe both rubber stoppers with alcohol pads and allow 30 seconds for evaporation

Bulletin of Experimental Biology and Medicine, 135(5), 495498