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The sections were then incubated in blocking solution (PBS supplemented with 2% Normal Goat Serum, 1% BSA, 1% Triton-X, .05% Tween-20, and .05% sodium azide) at room temperature for 1 h and incubated at 4C overnight with the following primary antibodies

Wani, K

2004;48(4):106176, viii

When circulating T 4 levels decrease, the pituitary gland increases its secretion of TSH, resulting in increased iodine trapping, as well as increased production and release of both T 3 and T 4

These findings highlight the intricate interplay between mechanosensitive and heat-sensitive channels in the PNS and CNS, suggesting Piezo2 as a potential therapeutic target for visceral pain treatment