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Different sites reduce local irritation, lower the risk of stacked injection-site reactions, and help you tell which compound is causing any reaction

To aliquot safely: Work in a clean environment, ideally with minimal air movement Use sterile technique throughout Prepare labeled sterile vials or containers in advance Draw from the master vial and dispense into individual containers Refrigerate all aliquots immediately The trade-off is that aliquoting introduces more handling steps where contamination can occur

Rotate injection sites: Alternate between thighs or arms to avoid irritation or scar tissue