Each sample was homogenized using a TissueLyser III (Qiagen) with 5 homogenization beads in 200 l of cold Tris lysis buffer (150 mM NaCl, 20 mM Tris, pH 7.4, 1 mM EDTA, 1 mM EGTA, 1% Triton X) containing a cocktail of protease (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 and 3, Sigma Aldrich), NaF and PMSF in DMSO
18 Participants received a weekly injection of a 6.0mg dose of survodutide, or placebo
It can be a good alternative when popular drugs like Ozempic and Wegovy are unavailable
Lab work is repeated every 3 months or sooner if needed
why should you buy from us not from other suppliers?We have a 28-years, have an independent research and development center, and the raw materials are from Europe, America and Thailand