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CRISPR-Cas9 is a construct consisting of a guide RNA (gRNA) that targets the genetic loci of interest and the Cas9 endonuclease enzyme, which functions as a pair of nucleotide scissors that cleave the DNA at the target siteeffectively generating a double-stranded DNA break for subsequent genome editing [51]

Cycle Protocols and Storage Requirements Standard Cycling Protocols Standard cycles use 8-12 weeks on with 3-6 weeks off (most commonly recommended), while short conservative cycles use 4-6 weeks on with 2-4 weeks off and repeat as needed

"Nasal drug delivery: Pharmacokinetic and pharmacodynamic considerations." Advanced Drug Delivery Reviews

Perna S, Guido D, Bologna C, Solerte SB, Guerriero F, Isu A, et al