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Koo BK, Kim LK, Lee JY, Moon MK

Ten thousand cells/well were plated in white 96-well plates (Table 1)

The success of such surface-based conjugation hinges on two critical criteria: (i) the availability of orthogonal chemical handles that selectively target specific amino acid residues without cross-reacting with other surface-exposed side chains, and (ii) coupling chemistries that preserve native protein folding and function