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Freezing causes ice crystal formation, which mechanically disrupts peptide structure and concentrates solutes in unfrozen regions (the "freeze concentration" effect)

Evidence suggests that fibronectin is a crucial factor in preserving the structural integrity of fetal membranes due to its role in extracellular matrix remodeling and the fetal membrane repair processes (55, 75)

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Both A-CDs and AA-CDs experienced a significant drop in fluorescence intensity due to Fe and Fe, whereas no obvious impact on their fluorescence intensity was observed with other cations