The membrane was incubated in blocking solution (5% (w/v) skim milk powder (LP0031, Oxoid), 0.1% (v/v) Tween20 (9,005645, Ampresco), 1x PBS (137 mM NaCl (BP358-212, Fisher), 0.027 mM KCl (7447-40-7, Anachemia), 10 mM Na 2 HPO 4 (SDB0487, Biobasic), and 2 mM KH 2 PO 4FF ) for 1 h, shaking, at room temperature
N.VillosladaP.Rodrguez-AntigedadA.MatuteC
The limit of quantitation was 0.17 mg/100mL
Specifications Scientific Name Bacteriostatic Water, USP CAS Number 7732-18-5 Molecular Formula HO Molecular Weight 18.02 g/mol Physical Form Sterile liquid solution Purity N/A Storage Store at 2025C (6877F) Storage & Handling Store per the product label and available manufacturer handling documentation, and follow standard laboratory protocols for handling
Do not take the following medications within 18 hours of an injection (before or after):MedicationDoseSildenafil (Viagra)20 mg to 100 mgVardenafil (Levitra)10 mg to 20 mgAvanafil (Stendra)50 mg to 200 mg Do not inject within 72 hours (3 days) of taking tadalafil (Cialis) 10 mg or 20 mg
