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J Neurosci 24(36):777988, PubMed PMID: 15356189 Kuroda J, Ago T, Matsushima S, Zhai P, Schneider MD, Sadoshima J (2010) NADPH oxidase 4 (Nox4) is a major source of oxidative stress in the failing heart

As we age, we produce less of this stomach hydrochloric acid, meaning older adults may be at risk of a B12 deficiency

Lane 1: Mouse kidney tissue lysate (40 g/Lane) Lane 2: Mouse liver tissue lysate (negative) (40 g/Lane) Lane 3: Rat kidney tissue lysate (40 g/Lane) Predicted band size: 26 kDa Observed band size: 22 kDa Exposure time: 25 seconds

I then discovered that the expiry date on the ampoules in my car was 2019 and they had obviously been exposed to extremes of heat and temperature fluctuations (but had been kept dark)